Abstract

The investigations were made on phosphorus metabolism in male rabbit brain under the different physical environments. The environmental factors were noise, cold and immobilization. The animals were exposed to 100-107 phon noise charactarized by wide octave band spectrum for 3 hours, to cold (-10°C±2°C) and immobilization for 90 minutes.During exposure to each condition, the animals were killed by air puncture. The brain was removed and separated rapidly into 8 parts; right and left telencephalon, diencephalon, the upper and lower part of mesencephalon, cerebellum, pons and medulla oblongata. The each part of brain was weighed rapidly and ground in ice-cold Trichloracetic-acid solution.Inorganic phosphorus was determined by Fiske and SubbaRow's method. The total phosphorus was determined as inorganic phosphorus after converting phosphorus in organic combination to inorganic phosphorus. Organic phosphorus was calculated by substracting the inorganic phosphorus from the total phosphorus.As the results, in the diencephalon and upper part of mesencephalon, inorganic phosphorus content was decreased significantly under the noisy condition, but it was not seen under the cold and the immobilized condition.The total and organic phosphorus content was not changed in exposure to each condition.

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