Abstract
Moringa oleifera leaf have been used for treatment of diabetes, in this work we studied pancreatic gene expression. The gene expression was done by PCR machine. The plant was harvested from garden within Madonna University and was identified in the Department of Pharmacognosy of the University by Dr P. Osuagwu. Each of the extracts was analyzed for bioactive components using Gas Chromatography–Mass Spectrometry (GC-MS). Male wistar albino rats (n=40) six weeks old weighing 150-250g were purchased from the animal farm of Madonna University Elele. From this work we observed that, there was significant increase in the expression of ADIPOQ gene in the diabetic rats treated with 300mg/kg of M. oleifera leaf (1.52±0.13), when compared with that of the non-diabetic rats (1.00±0.02). From this study, it could be inferred that M. oleifera leaf powder contains polyphenols that inhibit carbohydrate metabolism, reduced blood glucose level, enhance expression ADIPOQ genes.
Highlights
Diabetes mellitus is a disease characterized by hyperglycemia resulting from impairment of insulin secretion, release, transportation, stimulation and insulin action
From this table it could be observed that there is no significant difference in the pancreatic expression of the SNAP25, SNAP23, Kv2.1 and GLP-1 genes in all the rats treated in the experiment
From the results obtained from estimation of the pancreatic gene expression, there is a significant increase in the expression of ADIPOQ gene in the diabetic rats treated with 300mg/kg of Moringa oleifera leaf (1.52±0.13), when compared with that of the non-diabetic rats (1.00±0.02)
Summary
Diabetes mellitus is a disease characterized by hyperglycemia resulting from impairment of insulin secretion, release, transportation, stimulation and insulin action. Majority of type 1 diabetes is of the immune-mediated nature, the immune system cell (killer T-cells) attack the pancreatic tissue leads to the loss of beta cells of the pancreas, resulting in reduced insulin synthesis and secretion. GLP-1 RECEPTOR (glucagon-like peptide-1 receptor) gene produces instruction for producting GLP-1R, it is located at position 6 in human chromosome It is highly expressed in pancreatic beta cells, activated GLP-1R stimulates the adenylyl cyclase pathway which result in increase insulin synthesis and the release of insulin. RECTIFYING (Kv2.1/2.2) gene are highly expressed in pancreatic islet, it is involved in the regulation of glucose-induced insulin secretion in the beta cells. Intestinal cells produce incretins such as glucagon-like peptide-1, which functions to regulate blood glucose level by enhancing insulin production and regulation of appetite. The study was done to determine of pancreatic expression of ADIPOQ gene, Glucagon-like peptide-1 gene (GLP-1 RECETOR), RECTIFYING (Kv2,1/2.2) gene, Calcium voltage gated channel L-(CaV1.3), SNAP23/25 gene in the rats after treatment with pulverized Moringa oleifera leaf
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