Abstract
1. 1. ω-Oxidation of straight-chain fatty acids has been demonstrated with cell-free systems of guinea-pig liver. Cell-fractionation studies have shown that the 6000 × g supernatant fraction is the most active. 2. 2. Using sorbic acid amide, octatrienoic acid amide, or capric acid amide as substrate, formation of the corresponding dicarboxylic acod monoamide has been detected by paper chromatography, following incubation with the 6000 × g supernatant fraction of liver homogenate, together with NAD and the NADPGH 2-generating system. 3. 3. ω-Hydroxy-fatty acid has been found to be the intermediate compound and the hydroxylating reaction occurs in the microsomes. When [2- 14C]sorbic acid amide was incubated with microsomes, the formation of ε-hydroxy [2- 14C]sorbic acid amide was detected. Oxidation of ε-hydroxysorbic acid amide to muconic acid amide has been observed with a supernatant fraction of 105000 × g. 4. 4. The hydroxylating reaction requires NADPH 2 and oxygen. Catalase does not inhibit the reaction. The nature of the hydroxylating reaction is discussed.
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