Abstract
SUMMARYThree strains of cauliflower mosaic virus (CaMV) designated NVRS, CM4‐184 and PK caused respectively severe, moderate and mild reactions in turnip cv. Just Right plants and severe, mild and symptomless reactions in Brussels sprout cv. Fasolt plants.Chlorotic local lesions formed consistently in the leaves of young turnip plants when inoculated with each of the virus strains. Lesions were suitable for infectivity assay of crude and purified preparations of the virus.Three variants of the NVRS strain were isolated by single‐lesion transfer after treatment of the virus with nitrous acid (pH 5.0) and two variants were obtained after treating the virus in acetate buffer at the same pH. One of the variants (designated V3) caused symptomless infection in turnip and Brussels sprout plants.In cross‐protection tests, Brussels sprout plants infected symptomlessly with the PK, CM4‐184 or the V3 strains, subsequently resisted infection by the severe NVRS strain.
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