Abstract
A high percentage of isolated mesophyll protoplasts of Hyoscyamus muticus and Nicotina tabacum initially divided in the presence of comparatively high levels of phytohormones. However, shortly after culture initiation the requirement of the colonies for further proliferation changed. Rapid growth followed by prolific shoot formation was obtained when colonies initiated on high phytohormone levels were transferred after 14 days to the same medium but with reduced auxin concentration. It is proposed that such an early selection for morphogenetic cells may avoid problems inherent during prolonged callus proliferation.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Published version (
Free)
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
