Abstract
The present study was conducted on the microencapsulation of Lactobacillus acidophilus NCIM 5306 probiotic lactic acid bacteria. Sodium alginate, sodium alginate + 0.4% chitosan, sodium alginate with 2% inulin, and sodium alginate with 2% inulin + 0.4% chitosan coating were used as microencapsulated matrix material. The extrusion method was used for the formation of various micro encapsulates. The probiotic pomegranate juice was formulated using the obtained beads. The freshly prepared beads were first subjected to viability assay and then inoculated in pasteurized pomegranate juice at 1% quantity. The juice was stored at 4oC and further subjected to analysis for cell viability and sensory analysis. Response for cell viability was found discrete in various types of beads. The obtained results showed the use of 2% inulin as prebiotic in microencapsulation results in the prolonged period survival of the L. acidophilus NCIM 5306 up to six weeks.
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