Abstract
The isolation and identification of vitamin B12 produced in the cells of Klebsiella sp. No. 101 grown in a medium containing methanol as a sole carbon source were investigated. The B12-vitamers formed in the cells of the bacterium were extracted with hot ehtanol and purified by solvents extraction and ion-exchange cellulose column chromatography. The identity of the main component with 5,6-dimethylbenzimidazolyl cobamide coenzyme (DBCC) was demonstrated by paper chromatography, paper ionophoresis, absorption spectrophotometry of its coenzyme form, cyano form and nucleotide.
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