Abstract
The structure of a metabolite, C10H10O5 (I), obtained as a weakly toxic substance from the culture filtrate of Alternaria kikuchiana, was determined as 3,6,8-trihydroxy-3-methyl-3,4- dihydroisocoumarin (Ia). Though three tautomeric forms—lactol form (Ia), keto form (Ib) and enol form (Ic) were possible for (I), it was found that the compound (I) existed as the lactol form (Ia) both in a crystalline state and in acetone, chloroform and methanol solutions. This metabolite gave weak necrotic symptom to pear leaves, but stimulated the root elongation of rice and radish seedlings (ca. 20~30%) at 5 ppm and also showed synergistic activity with gibberellin A3 on the stem elongation of rice seedlings (ca. 30%).
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