Abstract
The saponin fraction of black medic trefoil (<em>Medicago lupulina</em>) roots was isolated and purified. Its hydrolysis afforded several aglycones that have been identified by spectral and chemical properties as medicagenic acid, hederagenine and soyasapogenols: B, C, D, E and F. They made up, respectively. 46.9 and 45% of total sapogenins isolated. Two-dimensional TLC of <em>M. lupulina</em> root saponins revealed fourteen compounds, two of which were medicagenic acid glycosides. The haemolytic, antifungal and allelopathic activities of <em>M. lupulina</em> and <em>M. media</em> roots are compared and discussed.
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