Abstract
Eleven strains of marine purple pigmented bacteria, isolated from coastal waters of Kinko Bay, Japan, were used in this experiment. These isolates were identified as Alteromonas luteooviolacea based upon their bacteriological characteristics, primarily their low G+C base ratios (40.4-42.4 mol %). The eleven strains of A. luteoviolacea were divided into two major groups according to pigment production, HCN production and ability to grow at 37°C.Nine strains produced two purple pigments and two yellow pigments which were separated by Sephadex LH-20 column chromatography. Two strains, 9K-V9 and 9K-V10, produced only two purple pigments. The main fractions of the purple pigments were identified as violacein based upon absorption spectra and color change reactions.All strains produced purple pigment on solid media containing alanine or proline as a sole carbon source. Upon addition of tyrosine to yeast extract agar medium, 9K-V9 and 9K-Vl0 increased violacein production, while all other strains produced brown pigment upon utilization of tyrosine. Violacein synthesis by 9K-V9 and 9K-V10 increased upon addition of tryptophan in concentration up to 200mg/100ml and decreased proportionally in the presence of higher concentrations of tryptophan.
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