Abstract
Besides nitrate-utilizing species and strains which also always are nitrite-positive, steadily nitrite-utilizing strains were found among the nitratenegatives of Cr. laurentii and D. hansenii. For other strains of these species, of T. famata, as well as of C. suecica and C. zeylanoides the nitrite media used seemed to provoke a genotypic adaptation (Figs. 1 and 2). This potential ability to utilize nitrite, may be of ecological significance to these arine occurring yeasts. Nine strains did grow with hydroxylamine as the sole nitrogen source in auxanograms. Only C. zeylanoides was able to give turbidimetrically measurable growth at the concentrations used, ranging from 0.133 to 3.33mm hydroxylamine. A phenotypic adaptation did occur. The hydroxylamine concentration in the culture medium was gradually reduced to zero during growth. Aldoxime formation in the culture medium was not a prerequisite for growth. Hydroxylamine reductase activity of cell-free enzyme preparations from C. zeylanoides has been proved.
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