Abstract
Due to growing population, there is an increasing demand of rice production but the productivity of rice is lessened day by day. To overcome this problem various biotechnological tools can be used for developing various rice varieties. However, the lack of a simple and efficient protocol for callus induction, embryogenic callus formation and quick plant regeneration in this cereal crop. In this study embryogenic calli from mature seeds of five indica rice varieties viz. Binadhan-5, Binadhan-6, BRRI dhan-48, BRRI dhan-58 and IR-64 were observed that is done in four different types of media composition. The highest callus induction were observed in media containing Sucrose as a carbon source. Among those varieties Binadhan-6 and BRRI dhan-48 showed highest rate of callus induction respectively. This study will be useful for selecting suitable callus induction medium for callus induction in future that will be useful for not only national but also international plant breeders for producing new variety and so on.
Highlights
Rice (Oryza sativa L.) is a monocot plant that belongs to the genus Oryza, family Gramineae (Poaceae) and tribe Oryzeae
Plant regeneration is a system that is essential for establishing an efficient application of biotechnology that can be utilized for crop improvement in future
In vitro regeneration from induced callus from mature embryos of five indica rice (Oryza sativa L.) varieties was investigated in this study
Summary
Rice (Oryza sativa L.) is a monocot plant that belongs to the genus Oryza, family Gramineae (Poaceae) and tribe Oryzeae It comprised two subspecies, Indica and Japonica [16]. For coping with the high rate of increasing population of the world the total rice production has to be intensified 50% within 2025 [8]. This goal cannot be accomplished by increasing the cultivation area under rice cultivation because of limitation of proper lands. Various researchers used different types of media for callus induction and regeneration [20],[21]. We hope that this study will be useful for local as well as for international plant breeders for improving the callus induction and regeneration efficiency in Rice in future
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