Abstract
Optimum conditions have been determined for growth of mink enteritis virus (MEV) in the feline kidney cell (FKC) lines. The S18and S36strains of MEV which grow to titres of 8192 to 32 786 haemagglutination (HA) units or 109·7–10·3TCID50by alternate cultivation for 22 passage in FKC with very different susceptibility. Mink with typical clinical symptoms of mink viral enteritis (MVE) eliminated intestinal mucosal cylinders from which the L12strain of MEV was isolated, which is capable of replication to 8192 HA units of 109·7TCID50. The S18, L12and S36strains of MEV grow to high titres and the inactivated virus vaccine induced a good immune response in mink. The S18and L12strains of MEV are the best strains for vaccine preparation and the L12strain is the most virulent for challenge tests.
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