Abstract
Pongamia pinnata is an oil producing tree species with multiple uses and considerable potential as a bioenergy crop. The present investigation has been carried out to assess the extent of genetic structure in a representative set of 111 individuals of P. pinnata encompassing seven populations as a prelude for utilization of promising and genetically divergent material in the breeding program. Molecular polymorphism was 67.18% with 10 Inter Simple Sequence Repeats (ISSR) between the individuals indicating modest levels of genetic variation in the P. pinnata germplasm collected. The within population variation based on ISSR polymorphism was 32.34% and polymorphism at the species level was 94.34%. Genetic differentiation between populations (GST= 0.61) was positively correlated with geographical distance. The data obtained indicated an immediate need for widening the genetic base of P. pinnata germplasm for proper characterization and extensive plantations of elite varieties to meet the biodiesel demands.
Highlights
Pongamia pinnata (Linn.) Pierre is an arboreal legume tree under family Leguminosae and high potential for oil
For Inter Simple Sequence Repeats (ISSR) analysis, the number of samples per population varied from 10 to 35 according to population size, the maturity of tree and the availability of the following conditions: leaves were sampled from trees at least 10 m apart, ± 6 m height and with a diameter at breast height ± 80 cm
The binomial matrix was used to calculate the level of polymorphism for each population
Summary
Pongamia pinnata (Linn.) Pierre is an arboreal legume tree under family Leguminosae and high potential for oil This tree is a potential for the biodiseal industry (Scott et al 2007). The mature tree can withstand water logging and slight frost and is highly tolerant to salinity and is common along seashores with its roots in fresh or saltwater (Kuashik et al 2007). It occurs naturally in lowland forests on limestone and rocky oral outcrops on the coast, along the edges of mangrove forests and along tidal streams and rivers. Bark, leaves, sap, and flower have medicinal properties and have an effect on a wide array of organisms including insects and pests, nematodes and molluses (Srinivasan et al 2003, Baswa et al 2001)
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