Studies on inclusion complex of 4-methyl 3-phenyl 2- thiocarbamoyl -3,3a dihydro pyrazolo[3,4c] pyrazole

Abstract

The compound, 4-methyl 3-phenyl 2-thiocarbamoyl -3,3a dihydropyrazolo (3,4c) pyrazole has been synthesized starting from 5-methyl-2,4-dihydro-3H pyrazol-3-one and benzaldehyde in its purest form and its inclusion complex has been prepared with β-cyclodextrin so as to increase its solubility and bio-accessibility in polar medium . The formation of inclusion complex has been ascertained by studying the changes in physical and spectral properties (UV, FT-IR, XRD, NMR etc). The thermodynamic stability constant and free energy of activation are studied to know whether the inclusion complex formation is thermodynamically allowed. Finally, antibacterial and antioxidant activity of the compound and its inclusion complex are studied. It is found that inclusion complex of 4-methyl 3-phenyl 2-thiocarbamoyl -3,3a dihydropyrazolo (3,4c) pyrazole with β- cyclodextrin is stable and increases antibacterial and antioxidant activities significantly.