Studies on immunocytochemical localization of inhibin-like material in human prostatic tissue: Comparison of its distribution in normal, benign and malignant prostates

V M Doctor,J P Aaveri,N J Arbatti,N A Sheth,M M Simha,A R Sheth

doi:10.1038/bjc.1986.85

Abstract

A specific antiserum has been generated against inhibin-like material (ILM) of prostatic origin. Using the immunoperoxidase technique, localization of ILM has been examined in a total of 114 prostates including normal (4 specimens), malignant (46) and hyperplastic (55) tissues. ILM positive immunocytochemical reactions were confined to the cytoplasm and not the nucleus of the prostatic acinar cells in the three categories of prostate, whereas the stroma showed negative reactions. The intensity of positive reactions decreased in the following order: Hyperplasia, incidental and moderately differentiated carcinomas, poorly differentiated carcinomas, whereas metaplasia and granulomatous prostatitis gave negative reactions for ILM. Using this experimental protocol, 200 non-prostatic tissue were found to be completely negative, demonstrating the specificity of the test for prostatic epithelium. These findings indicate a potential use of ILM as a marker of prostatic tissue.

Highlights

We report immunocytochemical localization of inhibin-like material (ILM) in prostatic tissue
Positive immunohistological staining for the presence of immunoreactive ILM was detected in epithelial cells of all prostatic tissues whether normal, benign on malignant
Within the cells that stained, the brown reaction product was dispersed throughout the cytoplasm but was absent in nuclei or nucleoli

Summary

Methods

The study was carried out on specimens from 311 subjects which included prostatic prostatic tissues from 114 patients and non prostatic tissues from 197 patients. Prostate specimens from 114 patients received at the Department of Surgical Pathology, Breach Candy Hospital and Research Centre and at the Department of Surgery, B.Y.L. Nair Hospital, Bombay (India), were included in the study. The specimens were obtained by trans-urethral resection, open prostatectomy on by perineal needle biopsy. Normal prostates were selected from autopsy specimens from subjects in their second and third decades and weighed

Results

Discussion

Conclusion