Abstract
Summary A new fractionation procedure is presented for the isolation of IgA from pooled normal human plasma. The method involves seven definable steps including ammonium sulfate precipitation of euglobulins, dialysis against Veronal buffer, a batch separation on DEAE-Sephadex A 50 followed by cold ethanol and ammonium sulfate precipitation, and semifinal resolution on TEAE-cellulose. The final key step utilizes immunospecific removal of residual traces of IgG on a bromoacethyl cellulose absorbent. From 350 ml of plasma 77 mg of purified IgA are obtained. The end product is pure by immunoelectrophoretic criteria and is representative of IgA in native plasma in terms of electrophoretic heterogeneity.
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