Abstract
1. 1. The amino acid content of hyaluronic acid fractions isolated from the rooster comb has been determined. It was found that five amino acids—serine, glycine, alanine, aspartic acid and glutamic acid—accounted for approx. 65% of the total amino acids present. The amounts of aspartic acid in each fraction were very similar. 2. 2. Expressed as a weight percentage, the amino acid content of the hyaluronic acid fractions was 0.35% and despite large variations in molecular sizes the amino acid contents per mole of hexuronic acid were very similar. 3. 3. During the purification of the hyaluronic acid there was a drop in the limiting viscosity number and also in the amino acid content. The protein(s) removed, however, had an amino acid composition that differed significantly from the component(s) that remained and were thought to be chemically distinct from the hyaluronic acid molecule as such. 4. 4. Alkali treatment of the hyaluronic acid fraction that constituted approx. 90% of the total present in the comb, decreased the serine content but did not significantly reduce the molecular size of the material. 5. 5. The significance of these data is discussed.
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