Abstract
Studies on Human DNA Polymerase ϵ and GINS Complex and Their Role in DNA Replication
Highlights
AUGUST 19, 2011 VOLUME 286 NUMBER 33 sivity
We examined the role of Pol ␦ and Pol ⑀ in cell cycle progression and fork movement by small interfering RNAs (siRNAs)-targeted depletion
We have described the isolation and characterization of the four-subunit human Pol (hPol) ⑀ complex and showed that its subunits are present in stoichiometric levels and that the holocomplex is a monomer
Summary
Polymerase; RFC, replication factor C; PCNA, proliferating cell nuclear antigen; RPC, replisome progression complex; hPol, hPCNA, hRFC, and hGINS, human Pol, PCNA, RFC, and GINS, respectively; aa, amino acids; IVTT, in vitro transcription/translation; nt, nucleotides; IdU, iododeoxyuridine; CldU, chlorodeoxyuridine. All Pol ⑀ preparations required PCNA and RFC for DNA synthesis on a singly primed M13 template, and hPol ⑀ appears to be more processive than hPol ␦ Both hPol ⑀ and hPol ␦ are stimulated by the GINS complex. These findings are consistent with the expectation that Pol ⑀ plays a critical role in governing leading strand synthesis
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