Abstract
In Japan there are two dangerous species : Mamushi (Agkistrodon blomhoffii) and Habu (Trimeresurus flavoviridis). Habu is found in the southwest islands of Japan. The cases of Habu snake bite in Amami Islands are about 300 per 150, 000 habitans a year and death rate is about 3 percent.The characteristic symptomes of Habu bite are hemorrhage and necrosis.The necrosis is severest in the parts rich in muscular tissues such as the leg. For the determination of Habu proteinase activity casein was used for substrate.The amount of split products remaining in solution was determined colorimetrically by means of the phenol reagent, calculated as micromoles of tyrosine.Crude Habit snake venom was fractionated by ammonium sulfate. Two fractions of Habu proteinase was obtained and each was designated as Hα and Hβ respectively.Hα proteinase was further purified by ammonium fractionation and calcium phosphate gel. Frac tion-5 obtained by those purification procedures is electrophoretically pure and has high proteinase activity. When guinea pig was given injection of Fraction-5, severe necrosis with hemorrhage was observed. The histopathological pictures were described.
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