Abstract
1. 1. An α-glucosidase was purified from Thaumetopoea pityocampa larvae (Lepidoptera Thaumetopoeidae) by means of chromatofocusing and hydroxyapatite chromatography. 2. 2. It was found to be homogenous on electrofocusing. 3. 3. Some characteristics of the enzyme were determined: mol. wt = 190,000; pI = 3.70; optimum pH = 6.0; K m for p- nitrophenyl-α- d- glucoside = 0.5 mM , for sucrose = 2.9 mM and for maltose = 0.87 mM. 4. 4. The enzyme hydrolyzes also maltose and the maltooligosaccharides series up to DP = 7 , turanose and kojibiose. 5. 5. Isomaltose, trehalose and amylose are not hydrolyzed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Comparative Biochemistry and Physiology -- Part B: Biochemistry and Molecular Biology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
