Abstract
A bacterial strain, HN-56, having an activity of D-glucose isomerization was isolated from soil, and was identified to be similar to Aerobacter aerogenes (Kruse) Beijerink. D-Glucoseisomerizing activity was induced when HN-56 was precultured in the media containing D-xylose, D-mannose, lactate, especially D-mannitol. Paper chromatography showed that the ketose formed in reaction system containing D-glucose was D-fructose alone. The optimum pH for the reaction was 6.5-7.0. Sulfhydryl reagents inhibit the reaction, but metal inhibitors affect little if any. With the washed living cells as enzyme source, only arsenate could accumulate D-fructose. In addition, the cells grown with D-mannitol and D-mannose showed no activity of D-xylose isomerase.
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