Studies on genes expression pattern of antioxidant enzymes and enzymes involved into the genetic information implementation in E.coli cells due to the antibiotic resistance against apramycin and cefatoxime

Oleg Fomenko,Mikhail Syromiatnikov,Svetlana Grin,Andrey Koshchaev,Nadezhda Pasko,Evgeny Mikhailov,D Fayzrakhmanov,B Ziganshin,R Shaydullin,F Nezhmetdinova

doi:10.1051/bioconf/20201700103

Abstract

The emergence of antibiotic-resistant bacteria is considered a serious problem. The resistance of bacteria against antimicrobial substances becomes important in the repair systems for damage to DNA and RNA molecules. The role of the antioxidant system in the development of bacterial resistance against antibiotics is not yet practically studied. The article studied the expression regulation of the genes of antioxidant enzymes and enzymes involved in the genetic information in E. coli cells with the antibiotic resistance against apramycin and cefatoxime. The study was conducted on bacterial cells resistant against these two antibiotics. The genes blaOXA-1, blaSHV, blaTEM, mdtK, aadA1, aadA2, sat, strA, blaCTX, blaPER-2, tnpA, tnpR, intC1 and intC1c were identified in bacterial cell case. This indicates the presence of plasmids in bacteria with these genes, which provide bacterial resistance to apramycin and cefatoxime. It was established that during the formation of cefotaxime resistance, there was a sharp increase in the expression of the Cu, Zn superoxide dismutase gene: in comparison with the control group, the representation of its transcripts increased 141.04 times for cefotoxime and 155.42 times for apramycin. It has been established that during the formation of resistance to the studied antibiotics in E. coli, an increase in the expression of the end4 and end3 genes is observed. There is tendency toward an increase in the number of transcripts of the pol3E gene observed in the formation of resistance against cefotaxime and apromycin.

Highlights

There is a wide circulation of microorganisms with multidrug resistance developed recently
Several researchers report that the main reason for the formation of bacterial resistance to many antimicrobial agents used is the production of R plasmids when conjugated from other bacteria [5,6,7,8], which contain antibiotic resistance genes
Antimicrobial resistance genes are present to varying degrees in most microorganisms, even in strains not previously exposed to antibiotics [9]

Summary

Introduction

There is a wide circulation of microorganisms with multidrug resistance developed recently. Several researchers report that the main reason for the formation of bacterial resistance to many antimicrobial agents used is the production of R plasmids when conjugated from other bacteria [5,6,7,8], which contain antibiotic resistance genes. They can be transmitted from one bacterium to another, forming resistance in the entire population. There is an important study of free radical oxidation processes and antioxidant protection in potentially pathogenic pathogens with the development of resistance to the main classes of antimicrobial substances and their compositions. Expression regulation of genes of antioxidant enzymes and DNA repair enzymes is one of the key steps in the control of free radical processes

Materials and research methods

Results and discussion

Conclusion