Studies on fermentation products and metabolism of long chain alkyl diamine in microorganisms.

Abstract

Ten bacteria capable of using 1,12-diaminododecane (DAD) as a sole carbon and energy source were isolated from soil samples.Four strains (K95, K55, K24, 110–2) were identified as Pseudomonas, four (DAD2–3, 10–23-A, 10–23-B, K61) as Nocardia and two (DAD2–1, 1994) as Corynebacterium on the basis of their taxonomic characteristics.Metabolic products such as 12-aminododecanoic acid, 12-hydroxydodecanoic acid, 1,10-decanedicarboxylic acid, sebacic acid, suberic acid, adipic acid and α-ketoglutaric acid were found in the culture broths of various bacteria. The yields of 12-aminododecanoic acid and 12-hydroxydodecanoic acid from DAD were as high as 55% and 20%, respectively.An amine dehydrogenase was found in cell-free extracts of Pseudomonas K95 and amine oxidases were detected in cell-free extracts of Nocardia 10–23-A.A biodegradation pathway for DAD is proposed.