Abstract
The expression of the 5-lipoxygenase and the 5-lipoxygenase activating protein (FLAP) genes in human tonsillar B cells and in a lymphoblastoid B cell line was demonstrated at the transcriptional level by the polymerase chain reaction (PCR) technique. Intact B cells produced very low amounts of leukotriene (LT) B4 and 5-hydroxyeicosatetraenoic acid upon stimulation with the calcium ionophore A23187 and arachidonic acid in comparison to those formed by sonicates of these cells. Incubation of intact lymphoblastoid B cells with the glutathione depleting agent, azodicarboxylic acid bis[dimethylamidel] (diamide), prior to the addition of the calcium ionophore A23187 and arachidonic acid, led to the formation of similar amounts of LTB4 as those produced by sonicated cells. These results indicate that the glutathione status is of importance for the activity of 5-lipoxygenase in B lymphocytes.
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