Abstract
To clarify the biochemical specificity of the barbital withdrawal, the concentrations of glycolytic metabolic intermediates and the activity of adenylate cyclase (AC) in the rat brain were examined. Male S.D. rats were used. Barbital dependence was aquired in rats by the DAF method. PTZ (80 mg/kg, s.c.) treated or barbital-withdrawn rats were sacrificed by decapitation. The brain was dissected into the cerebral cortex, brain stem and cerebellum as rapid as possible. Concentrations of glycolitic metabolic intermediates were measured by photometrically. The AC activity was increased at PTZ seizure. The concentration of glucose in the cerebral cortex and brain stem was decreased, however, the concentration of glucose-6-phosphate was increased. In cerebellum, the concentrations of glucose and glucose-6-phosphate were not changed by PTZ-seizure, but the concentrations of glycogen and lactate were not affected. The concentration of CP was not significantly decreased. The concentration ATP was decreased in the brain stem, and AMP was decreased in three portions. These changes in PTZ seizure were completely different from barbital-withdrawal. These results suggested that the changes induced by barbital withdrawal were probably specific to the barbital-withdrawal, not non-specific change caused by convulsion.
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