Abstract
Experiments were performed to determine some of the factors responsible for the increase in the fraction of inorganic 131I which always accompanies the release of 131I-iodinated amino acids from thyroid protein during pancreatin digestion of labeled thyroid homogenates. When H2O2 (8 X10-6-8 X10-2M) was added to a pancreatin digest of 131I-labeled rat thyroids, a large increase in inorganic 131I was observed, while at the same time the proportion of 131I present as iodothyronine greatly decreased. There was only a minor effect on the 131I-iodotyrosine fraction. The deiodinating effect of H2O2 could be greatly inhibited by8.8XlO-3M methylmercaptoimidazole (MMI), indicating that an oxidative step must be involved in the deiodination process. These observations suggested that the iodide increase that occurred during pancreatin digestion might also be inhibited by a reducing agent such as MMI. Various concentrations of MMI were tested for such an effect, and it was observed that both the yield of 131I-iodothyr...
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