Abstract
Summary The experiments carried out on the oxidation of butyric acid by A. niger indicate that the butyric acid oxidation in vitro by molds can be believed to take place according to the beta -oxidation theory as well as according to the dehydrogenation mechanism, as the presence of acetoacetic acid in the oxidation products of butyric acid might be regarded as positive proof in support of the former hypothesis, and the development of slight unsaturation being indicative of the latter. The pH optimum for the oxidation of butyric, β -hydroxybutyric, and crotonic acids has also been determined. With these preliminary results in hand it has been possible to carry out a more detailed and quantitative study on the mechanism of butyric acid decomposition by the mold and this will form the subject of our next communication.
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