Abstract
1. 1. The metabolic transfer of nucleotide label from [ 32P]s-RNA to microsomal RNA has been followed over various periods of time. Cofactors and s-RNA have been replenished during incubation with rat-liver microsomes. 2. 2. It has been demonstrated that both ATP and GTP stimulate the transfer reaction, but in an independent fashion. These results have been interpreted by assuming that only s-RNA molecules carrying the intact terminal structure are able to interact, provided GTP is present. It is furthermore suggested that ATP restores the structural integrity of the s-RNA terminus, although a more specific function for ATP so far cannot be excluded. 3. 3. A tentative representation of the mechanism of the interaction between s- and m-RNA is given, taking into account these and earlier data. A cyclic exchange of an oligo- or polynucleotide is proposed, comprising the terminal part of the s-RNA chain. Possible implications for the process of protein biosynthesis in rat-liver microsomes are discussed.
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