Abstract
Cytochrome P4501A (CYP1A) has been investigated extensively in the liver of adults of many different fish species. This study reports the presence and elevation of CYP1A measured as 7-ethoxyresorufin O-deethylase (EROD) activity and protein amount (Western blot — semi-quantified by image analysis) in larval, juvenile and adult turbot ( Scophthalmus maximus) after exposure to organic contaminant and model inducers. Basal EROD activity was not detectable in embryos, but increased from 3 day larvae (whole body 13 500g supernatants) through 90 day juveniles (hepatic 13 500g supernatants) to adults (hepatic microsomes), respectively in pmol/min/mg protein (± SEM), 0.57 ± 0.06, 10.8 ± 2.4 and 12.3 ± 4.7. Exposure to 5 ppb benzo[a]pyrene (B[a]P), 1 ppb γ-hexachlorocyclohexane (lindane) and 25 ppb B[a]P caused respectively, 3-, 6- and 2-fold elevation of EROD activity in 4 and 9 day larvae and juvenile stages. Image analysis of Western blots of juvenile samples detected a 3-fold difference in CYP1A protein whereas EROD activity of the same sample varied 4-fold. The results confirm the potential of using CYP1A induction as a biomarker for impact by organic pollution on early developmental stages of fish.
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