Abstract
A new ECTEOLA cellulose ion exchange chromatographic procedure for the fractionation of glycosaminoglycans has been applied to corneal polysaccharides. About 80% of the total keratan sulfate was recovered in a pure state; this keratan sulfate was found to contain more than one sulfate per hexosamine moiety. The remaining 20% of the keratan sulfate contained less than one sulfate per hexosamine moiety and appeared in the chromatogram together with the bulk of the galactosaminoglycans, which were found to be chondroitin 4-sulfates containing 0·22–0·41 sulfate per hexosamine moiety.
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