Studies on copolymerization of N-isopropylacrylamide with poly(ethylene glycol) methacrylate

Abstract

The paper describes the preparation and characterization of cross-linked homopolymers and copolymers of N-isopropyl acrylamide (NIPAAm) with poly(ethylene glycol) methacrylate (PEGMA, M n = 526 g/mol). Several copolymer samples were prepared by taking varying amounts of monomers i.e. NIPAAm and PEGMA in the initial feed using hydrophilic (IRGACURE-2959) and hydrophobic (DURACURE-1173) photoinitiator. In order to investigate the effect of reaction conditions, copolymers were prepared below or above the lower critical solution temperature (LCST) using water or water:ethanol (50:50) as solvent and by varying the amounts of cross-linker. Hydrogels prepared under varying reaction conditions were characterized for its swelling behaviour (using optical microscope), phase transition temperature (using DSC) and morphology (using SEM). As expected LCST increased from 35 to 39 °C as PEGMA content in copolymers increased from 1 to 20% (w/w). However, the morphology of hydrogels was found to be independent on the reaction conditions. Copolymer films having an optimum combination of swelling and performance properties were evaluated as switchable cell culture membranes. Hepatic cancer cell lines (Hep G-2) was used to study the cell growth and detachment. Cell growth and detachment were found to be dependent on the copolymer composition. Cell viability was found comparable to trypsin which also supports application of these films as cell culture membrane.