Studies on comparison of metabolites in urine between deprenyl and methamphetamine administration. I. Metabolites in monkey urine.

Abstract

Metabolites in the urine after administration of deprenyl and methamphetamine were compared. Determination of deprenyl, N-desmethyldeprenyl, methamphetamine, p-hydroxymethamphetamine, amphetamine and p-hydroxyamphetamine in the urine was determined by use of solid extraction, trifluoroacetylation and bis (trimethylsilyl) acetamide treatment, and gas chromatography-mass spectrometry. This method was applied to the determination of metabolites in the urine of monkey administered subcutaneously at 0.2 mg/kg of deprenyl hydrochloride or methamphetamine hydrochloride. Deprenyl and desmethyldeprenyl were detected in the urine only within 12 h after deprenyl administration. Even after their disappearance, the prolonged excretion of methamphetamine and amphetamine, metabolites of deprenyl, were found until 48 h after deprenyl administration. As a result, it was suggested that deprenyl or desmethyldeprenyl in the urine seemed to be used as a marker for the discrimination between deprenyl and methamphetamine use. However, the faster disappearance of deprenyl and desmethyldeprenyl should be taken into consideration.