Studies on changes in microstructure and proteolysis in cow and soy milk curd during fermentation using lactic cultures for improving protein bioavailability

Abstract

Cow milk curd was prepared using 2% v/v of Streptococcus thermophilus DG1 and a mixed culture (0.5:1.5v/v) of S. thermophilus DG1 and Lactobacillus plantarum and incubating at 37°C for 16h. Soy milk curd was prepared using different ratios of lactic cultures as stated earlier and also a mixed culture containing S. thermophilus DG1, L. plantarum and Leuconostoc mesenteroides sub spp. mesenteroides in the ratio 1:1:1v/v along with beet pulp (2% w/w) and incubating at 37°C for 18h. This improved functional and probiotic properties of curd. Structural changes in curd samples during fermentation were observed by Scanning Electron Microscope (SEM). Soy milk curd showed loosened structure. The degradation of proteins into peptides and amino acids were evaluated by SDS PAGE and amino acid analysis. Maximum production of amino acids i.e. cystine, histidine and asparagine were observed in both the cow and soymilk after fermentation.