Abstract
Purified gangliosides prepared by the tetrahydrofuran procedure were chromatographed on thin layers of silica gel, using a solvent system of chloroform–methanol −7% ammonia, 55/40/10, v/v/v. A resorcinol detection spray revealed a complex reproducible pattern of five major and several minor components which was similar to that observed in crude lipid extracts of brain. The major components were isolated by a preparative thin-layer technique as a slow fraction containing three components, an intermediate, and a fast fraction. Analysis gave N-acetylneuraminic acid contents of 33.4%, 26.6%, and 15.8% for the slow, intermediate, and fast fractions and the following molar ratios of ceramide:glucose:galactose:hexosamine:N-acetylneuraminic acid: slow, 1.0:0.9:3.2:1.0:2.6; intermediate, 1.0:0.8:2.7:0.8:1.7; and fast, 1.0:0.8:2.2:0.7:0.8.
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