Abstract
The preparation and stability of lysozyme labeled with 3H or 131I were studied. 131I-lysozyme was found to be convenient for investigation on biotransformation of the enzyme. The transport of 131I-lysozyme across rat intestine in vitro was studied using rat everted jejunum. The transport of lysozyme from the mucosal to serosal side was confirmed by several methods such as TCA-precipitation, immunoprecipitation and enzymatic activity assay. The intestinal absorption of 131I-lysozyme in vivo was studied in rats paying attention to the lymphatics and the portal vein as the route of absorption. The absorption rate of immunoprecipitable 131I via lymphatics was 0.07% of the dose during the first 6 hr after intraintestinal administration of 2 mg/kg of 131I-lysozyme. The absorption rate of immunoprecipitable 131I via portal vein was calculated as 1.99% of 131I-lysozyme dosed, by pharmacokinetic analyses on the concentration of immunoprecipitable 131I in serum. Absorption via lymphatics was 3.24% of the total immunoprecipitable 131I absorbed via both routes. The main route in intestinal absorption of 131I-lysozyme following intraintestinal administration was suggested to be the portal vein, not lymphatics.
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