Studies on Auxin Protectors. VII. Association of Auxin Protectors With Crown Gall Development in Sunflower Stems

Abstract

Mature sunflower internodes contain only very low concentrations of auxin protectors. Wounding such internodes results in a temporary increase of protector substances. Inoculating the wounds with a virulent strain of Agrobacterium tumefaciens Conn results in a dramatic rise in protector substances, particularly the very high molecular weight protector "A", which continues as the tumors develop. The levels attained in tumor tissue are comparable to those normally encountered in meristematic tissue. Since the auxin protectors are antioxidants which inhibit the peroxidase-catalyzed oxidation of IAA, and in view of the findings by other workers that the meristematic tissues are maintained in a relatively reduced state, the presence of such large quantities of protector substances in the tumor tissue could explain the physiological and morphological similarity of tumor tissue to young meristematic tissue.