Abstract
Some physicochemcial properties and amino acid compositions of the Aspergillus melleus proteinases were investigated. 1) Semi-alkaline proteinase from Aspergillus melleus : The molecular weight was 30300 using the Svedverg's formula, based upon the values of the sedimentation coefficient (S020, w=2.98S), the diffusion coefficient (D020, w=8.75×10-7 cm2/sec), and the partial specific volume (V=0.728). The enzyme contains 16.35% of nitrogen and is composed of 314 residues of amino acid ; Asp. 36, Glu. 18, Ser. 37, Thr. 25, Gly. 45, Ala. 46, Val. 33, Leu. 21, Ileu. 15, Pro. 9, Phe. 7, Tyr. 11, Trp. 2, Arg. 2, Lys. 5, His. 2, (amide-NH3)16. 2) Alkaline proteinase from Aspergillus melleus : The molecular weight was 27900 using the Svedverg's formula, based upon the value of the sedimentation coefficient (S020, w=2.85S), the diffusion coefficient (D020, w=9.05×10-7 cm2/sec), and the partial specific volume (V=0.726). The enzyme contains 16.10% of nitrogen and is composed of 282 residues of amino acid ; Asp. 33, Glu. 16, Ser. 33, Tyr. 17, Gly. 45, Ala. 39, Val. 22, Leu. 16, Ileu. 10, Pro. 6, Phe. 5, Tyr. 15, Trp. 3, Arg. 5, Lys. 12, His. 5, (amide-NH3)16.
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