Abstract
Apple insoluble pectins were extracted by pectolytic enzymes alone and combined with side-chain degrading enzymes or endo-glucanase. The highest yield of pectic material was obtained with the combination pectinlyase plus endo-glucanase, showing the existence of a connection between rhamnogalacturonan and xyloglucan. The extracts were fractionated on Sephacryl S 500 and DEAE Sepharose CL-6B. Methylation analysis of high-molecular-weight material coming from the ‘hairy’ regions showed the presence of highly branched arabinans and arabinogalactans of type I and II side-chains, and of terminal xylose residues linked to the rhamnogalacturonan backbone.
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