Abstract
Toxic Cyanobacteria (blue-green algae) bloom in eutrophic, freshwater lakes and ponds in New England and have caused environmental, health, legal and recreational problems over the past 15 years. Although several species have been implicated with animal kills and water fouling, a common offender was Aphanizomenon flos-aquae. Representative strains of A. flos-aquae bloom in New Hampshire intermittently, in both toxic (aphantoxins) and non-toxic forms. Research has focused on methods of: a) toxin accumulation from natural blooms and laboratory cultivation, b) toxin assay, using the mouse bioassay and a modified fluorometric technique developed for paralytic shellfish poisons, c) toxin characterization and purification, using solvent separation and molecular weight filters, and d) testing active extracts on nerve and muscle preparations to determine the specific sites and modes of action of aphantoxins.
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