Studies on antiviral antibiotics from streptomyces. XII. Further studies on the antiviral antibiotic, myxoviromycin.

Abstract

Crude myxoviromycin hydrochloride was further purified by means of carbon chromatography according to the description of Carter, and crystalline reineckate was induced. The recrysterized reineckate was converted to the pure hydrochloride. The antibacterial activity of the hydrochloride was quite low. In a concentration of 62.5 mcg/ml it inhibited B. subtilis (PCI 219) and S. lutea (Hata), and in 250 mcg/ml inhibited S. lutea (Hosoya) on an agar plate. Myxoviromycin hydrochloride showed a minimal inhibitory concentration for growth of MNI group viruses in vitro as follows: 8.8 mcg for Influenza A (PR 8), 1.7 mcg/ml for Influenza B (Lee), 4.3 mcg/ml for Sendai virus (Fushimi) and 8.2 mcg/ml for NDV (46-9674). Myxoviromycin hydrochloride had a delayed toxicity, the LD50 was 20.4mg/kg in ddD mice by subcutaneous injection. In the hydrolysate of the antibiotic, three ninhydrin positive substances were recognized in its paperchromatography, and two spots of them were corresponded to β-alanine and valine respectively, but the rest was not identified.