Studies on anti-inflammatory activity of herbal extract mixture using inflammation induced human keratinocytes (HaCaT) cells

Abstract

Inflammation is a general name for reactions occurring in several types of tissue injuries, infections, or immunologic stimulation as a defense against foreign or altered Endogenous substances. This comprises of a series of changes of the terminal tissues, which tend to eliminate the injurious agents and to repair the damaged tissue. Chemokines are a family of small proteins secreted by various cell types that play a role in the immune cell infiltration into inflammatory or infectious sites [1, 2]. Skin inflammation is closely associated with the production of Th2 chemokines [3]. These chemokines released from keratinocytes bind to and attract CCR4-positive Th2 cells into inflammatory tissues. It was reported that GJBRH inhibited the production of inflammatory cytokines by dermal endothelial cells. This study aimed to investigate whether GJBRH has inhibitory effects on TNF-α and IFN-γ-induced Th2 chemokine production and to identify the underlying molecular mechanisms in HaCaT human keratinocyte cell line. The present study was planned to evaluate the Herbal Extract sample for anti inflammatory activity using human skin keratinocytes