Abstract
Fluorometric determination of tripelennamine hydrochloride (I) in mixed anti-cold pharmaceutical preparations was studied, and a selective assay method with high sensitivity was established, The fluorescent substance of this reaction was extracted and isolated as an iodide. This substance, mp 150-151°, has the formula C14H15N2I, and its chemical structure was found to be 1-benzyl-2, 3-dihydroimidazo1o[1, 2-α]pyridinium bromide, and the fluorescence is produced by ring formation to a quaternary ammonium compound, accompanied with separation of dimethylamino group, from the result of a moderate oxidation of I by BrCN. This assay method is as follows : Tripelennamine is extracted with petroleum ether from the sample solution containing about 4 mg of I and the extract is evaporated with small amount of citric acid. The residue is dissolved in EtOH to make 1000 ml. To 4 ml of this ethanolic solution, 10 ml of pH 8 buffer solution and 4 ml of 2.5% BrCN solution are added and the mixture is heated at 50° for 30 min. The fluorescence of cold solution is determined at 415 mμ, exciting at 340 mμ.
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Schedule a callMore From: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
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