Abstract
Application of the radioallergosorbent technique (RAST) as a method for determining allergenic activity in horse epithelia extracts was investigated. Two variants of RAST are described: a direct and an indirect RAST allergen assay. Different batches of commercial horse epithelia extracts showed large variations with regard to allergenic activity. Gel chromatographic separation of the extracts showed that they contained a wide molecular weight spectrum of allergenic components in the range 10,000–75,000 g/mol with large variations between different extracts as to the composition of allergenic components. Large variations were also found between different patients with regard to the pattern of components in the extracts to which the patients were allergic. Irrespective of whether a skin test or RAST is used as a test method, the measure of the allergenic activity of an extract is dependent on the reagin pattern of the patients selected for the test. The extracts contain several components without allergenic activity. Results from fractionation of allergen extracts by ion-exchange chromatography indicate that this method might be suitable for isolating allergenic material in horse epithelia. It is suggested that RAST should be used for monitoring preparation and quality control of allergenic extracts.
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