Abstract
AbstractA quartz plate, whose surface was treated with octyltrichlorosilane (OTS), was used as a slab optical waveguide (SOWG) in studying the absorption of hemoglobin by measuring the in situ adsorption spectrum. More hemoglobin is adsorbed on a hydrophobic surface than on a hydrophilic surface. The amount of adsorption increases depending on the time required for surface processing and the solvent concentration. Adsorption of hemoglobin onto the surface did not reach equilibrium even after more than 1 hour. In the case of a hydrophilic surface, the light absorption becomes almost constant within several minutes. The hemoglobin once adsorbed onto the surface persists even if buffered solvent is injected. It is therefore confirmed that hemoglobin is reduced by a reducing solution. © 2003 Wiley Periodicals, Inc. Electron Comm Jpn Pt 2, 86(8): 61–66, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecjb.10125
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