STUDIES ON ADRENAL ENUCLEATION

Abstract

In the first, Na22Cl, Rb86Cl and Cs137Cl were subcutaneously given in a dose of 1μc/ 0. 1cc to male mice of dd strain weighing 2025g following right adrenalectomy and left adrenal enucleation (UAE) or bilateral adrenalectomy, and the excretions and distribution of each in the body was investigated and regenerating adrenal glands were histologically studied. Next, using rats weighing about 200g serum Na and K concentration following UEA and effects of various hormones upon it were investigated with the following results.1) Within 1 week after UAE, Na22 excretion rate was in inverse relation with those of Rb86 and Cs137, but all returned to the, normal after 1 week. Within 1 week the concentrations of Rb86 and Cs137 in various organs were significantly increased but returned to normal after 2 weeks.2) Histologically, regeneration of mouse adrenal gland observed at 4th day after UAE and regenerating cell was generally presented fascicular arrangement. Adrenal glands were restored to normal almost at 18th day after UAE, but zonal formation is not clear. At 35th day, its state is like to adrenal medullectomy.3) Serum Na and K concentration were returned to normal within 12 weeks after UAE, and it was not related to zonal formation.4) As to the influences of hormane administration to serum Na and K concentration, definit relation was not gained on adrenal corticoids and ACTH administration, administration of adrenalin and growth hormone produced no remarkable change, DOCA administration increased serum Na concentration.From the results of present experiments, histological finding is not keep with the restoriction of mineral metabolism. There is a scope to criticized to so-called zonal theory, that is, early regenerating adrenal gland cells after UAE have mineral and gluco metabolism functions.