Abstract
The ordinary mitochondrial preparation obtained from rat brain by differential centrifugation was subfractioned with a technique of gradient centrifugation in a medium of sucrose-Ficoll. Since ATPase activities of the various subfractions differ markedly in their response to certain agents and physical change, there would appear to be at least two distinct varieties of the enzyme in the ordinary mitochondrial preparation. One is present in the mitochondrion itself, and the other appeared to be bound to membranous components of the cells. The present findings with pure mitochondrial preparations would not vitiate the previous conclusion that the ATPase of mitochondria from brain differs in important respects from those of other tissues.
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