Abstract
A procedure for the quantitative analysis of oryzalides and oryzalic acids in leaves of rice cultivars was established by using GC-MS-selective ion monitoring (GC-SIM), 17-deuterated oryzalide B being used as an internal reference. The total contents of four compounds of oryzalides and oryzalic acids in the leaves at three different growth stages of Norin-27, i.e., seedling, tillering, and ripening, showed values of 5.3, 17.9, and 37.9 μg/g, respectively. The contents of oryzalides and oryzalic acids in each organ, i.e., leaf, stem, ear, and root, showed values of 35.0 and 1.7 μg/g for the former two, respectively, and were not detectable for the latter two. A preliminary analysis of Norin-27 and Rantai emas leaves inoculated with an incompatible and compatible strain of Xanthomonas campestris pv. oryzae showed a greater accumulation of oryzalides and oryzalic acids, especially in the lesion area, than the figures for healthy leaves. Mechanical injury by a needle-bundle also increased the total amounts.
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