Abstract
A protein (P8) found in cultured mammalian cells has been highly purified by DNA—cellulose chromatography alone. The protein is quite abundant, especially in human diploid fibroblasts, is readily extractable at low-salt concentration, and has an isoelectric pH close to neutrality. Its synthesis is not coupled to that of DNA, but it accounts for a greater proportion of the total soluble protein synthesis in growing than in resting cells. Among the proteins identified after DNA—cellulose chromatography, only P8 has affinity for single-stranded DNA but not for double-stranded DNA. It appears to have properties different from those of other DNA-binding proteins that have been described.
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