Abstract
1. 1. Giant ragweed pollen extract was subjected to digestion by freshly assayed pancreatin in calibrated dialyzing sacs. Digestion was also attempted with a highly active pepsin. In some cases as many as nine daily additions of enzyme were made to insure complete proteolysis. The digestion was followed by prolonged dialysis. 2. 2. All digests showed a marked loss of activity which was not accounted for by dialysis alone. Nitrogen analysis indicated that digestion was complete in the case of tryptic digestion. 3. 3. We cannot be certain that it was complete in the case of peptic digestion, because the membranes were not sufficiently permeable to permit all of the products of peptic activity to dialyze away. 4. 4. There is a small but constant amount of activity in this pollen which is unaffected by proteolytic digestion.
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